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KMID : 0104920200290010018
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Journal of Hawhwa Medicine
2020 Volume.29 No. 1 p.18 ~ p.25
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Retardation of axonal regeneration in the sciatic nerve after injury in streptozotocin-induced diabetes
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Hwang Jin-Yeon
Kim Ki-Joong
Namgung Uk
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Abstract
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Objective: The goal of this study is to investigate whether peripheral axonal regeneration is affected by diabetes in experimental animals.
Method: Sprague Dawely rat was injected with 45~50 mg/kg of streptozotocin (STZ) to generate an animal model of diabetes. Three months after STZ injection, sciatic nerve (2 cm length) was removed and the same length of nerve segments from STZ-injected animal or from control animal (CTL) was transplanted into STZ - injected animals (STZ - STZ and STZ - CTL respectively). Similarly, sciatic nerve segments from STZ - injected animal or from control animal were grafted into the control animals (CTL - STZ and CTL - CTL respectively). All animals were sacrificed 2 weeks after transplantation. Sciatic nerve sections were prepared and subjected to immunofluorescence staining analysis.
Results: Immunofluorescence staining for NF - 200 showed that distal elongation of regenerating axons reached 40~80% of proximal neve in both CTL - STZ and CTL - CTL groups. However, distal elongation in both STZ - STZ and STZ - CTL groups were 20~60% of proximal nerve. Furthermore, measurement of axonal regeneration after immuno - staining with SCG10 showed that the scores of distal elongation relative to proximal nerve were 50~90% in CTL - CTL and CTL - STZ groups and 10 - 60% in STZ - CTL and STZ - STZ.
Conclusions: Our data showed that the levels of axonal regeneration were not affected irrespective of whether they were from STZ- or CTL graft, but were greatly reduced when the nerves were transplanted into the STZ host.
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KEYWORD
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Diabetes, Axonal regeneration, transplantation, Streptozotocin
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